摘要：

为考察厌氧氨氧化菌在不同环境下对盐度的响应情况，以及生物量对厌氧氨氧化菌高盐抑制的缓解效果，研究未添加盐度和添加不同盐度的SBR反应器的脱氮性能，确定起到促进及抑制作用的盐度阈值。通过批次实验考察低盐度对35、15 ℃厌氧氨氧化污泥的影响，以及不同污泥量（4、6、8、10 g/L）在高盐抑制条件下对基质的降解能力。结果表明，低于5 g/L的盐度对中温和低温系统中的厌氧氨氧化菌均有促进和稳定作用。尤其在低温条件下，该盐度可提高厌氧氨氧化菌的亚硝氮还原能力，盐度达到14 g/L后出现明显抑制。此外，生物量为10 g/L的厌氧氨氧化颗粒污泥对盐度冲击具有一定耐受度。单位盐度负荷下的比厌氧氨氧化活性（SAA）在不同生物量时的变化规律相似。当单位生物量的盐度增至1.8 kg/kg时，SAA为初始数值的50%。明确了不同环境条件下盐度对厌氧氨氧化菌的响应情况及生物量的缓解策略，可为厌氧氨氧化菌在含盐废水脱氮工艺中的应用提供参考。

关键词: 厌氧氨氧化, 盐度, 低温, 颗粒污泥

Abstract:

To investigate the response of anammox bacteria to salinity in different environments and the effect of biomass on mitigation of high salt inhibition of anammox bacteria， the denitrification performances of SBR without and with different salinity additions were investigated to determine the salinity threshold for promotion and inhibition. The effects of low salinity on anammox sludge at 35 and 15 ℃ and degradation ability of different biomass（4， 6， 8 and 10 g/L） on the substrate under high salinity inhibition conditions were investigated by batch experiments. The results showed that salinity below 5 g/L promoted and stabilized the anammox bacteria in both medium and low temperature systems. In particular， the salinity improved the nitrite degradation ability of anammox bacteria under low temperature， and significant inhibition occurred after salinity reached 14 g/L. In addition， the anammox granular sludge with a biomass of 10 g/L was tolerant to salinity shock. Specific anaerobic anammox activity （SAA） per unit salinity load had similar pattern at different biomass. When the salinity load per unit biomass increased to 1.8 kg/kg， the SAA was 50% of the initial value. The response of salinity to anammox bacteria under different environmental conditions and the mitigation strategy of biomass were clarified， which can provide a reference for application of anammox bacteria in saline wastewater denitrification process.

Key words: anaerobic ammonia oxidation, salinity, low temperature, granular sludge

中图分类号: 

• X703.1