黄铁矿驱动混养反硝化体系的关键运行参数优化

doi:10.19965/j.cnki.iwt.2025-0430

摘要/Abstract

摘要：

水体富营养化问题日益加剧，而传统脱氮除磷工艺在低碳源条件下效率低、成本高。为此，在批次反应器中构建以黄铁矿驱动的混养反硝化体系（PMD），系统探讨黄铁矿投加量、C/N、pH与温度对其脱氮除磷性能的影响。结果表明，黄铁矿投加量为60~150 g/L，C/N为3时，体系脱氮除磷效果最优，总氮和总磷去除率分别达91.73%和99.85%。黄铁矿过量会引发钝化效应和亚硝氮积累，抑制脱氮；C/N过高则抑制自养反硝化，影响除磷。中性（pH=7）与中温（25~35 ℃）条件下协同效能最强。偏酸性抑制酶活性、诱导水解，偏碱性则阻碍电子传递、激活硝酸盐异化还原反应，低温抑制酶促反应速率，高温则破坏酶结构稳定性，导致脱氮效率下降。污泥分析表明，黄铁矿投加量增加虽提升了污泥浓度，但高投加量会造成无机沉积，降低传质与系统稳定性。研究明确了PMD工艺的关键运行参数，为同步脱氮除磷技术的工程化应用提供理论支撑。

关键词: 黄铁矿, 混养反硝化, 污水处理, 同步脱氮除磷

Abstract:

With the intensification of water eutrophication, traditional nitrogen and phosphorus removal processes face challenges such as low efficiency and high cost under low-carbon conditions. In this study, a pyrite driven mixotrophic denitrification(PMD) system was constructed in batch reactors. The effects of pyrite dosage, C/N ratio,pH and temperature on nitrogen and phosphorus removal were systematically investigated. Results showed that optimal performance was achieved at pyrite dosage of 60-150 g/L and C/N ratio of 3, with total nitrogen(TN) and total phosphorus(TP) removal rates reaching 91.73% and 99.85%, respectively. Excessive pyrite addition induced passivation and nitrite accumulation, inhibiting denitrification, while overly high C/N ratios suppressed autotrophic denitrification and reduced phosphorus removal. Neutral pH(pH=7) and moderate temperatures(25-35 ℃) provided the best synergistic performance. Acidic conditions inhibited enzyme activity and induced sludge hydrolysis, whereas alkaline conditions hindered electron transfer and triggered dissimilatory nitrate reduction. Low temperatures reduced enzymatic activity, while high temperatures damaged enzyme stability, both leading to efficiency loss. Sludge analysis revealed that increased pyrite raised sludge concentration but excessive dosage caused inorganic accumulation,reducing mass transfer and system stability. This study clarified key operational parameters for PMD and provided theoretical support for its engineering application in simultaneous nitrogen and phosphorus removal.

Key words: pyrite, mixotrophic denitrification, sewage disposal, simultaneous nitrogen and phosphorus removal

中图分类号:

X703.1

徐黄欢, 武英, 林旭萌, 陈炳江, 周启帆, 薛罡. 黄铁矿驱动混养反硝化体系的关键运行参数优化[J]. 工业水处理, 2026, 46(4): 164-172.

Huanghuan XU, Ying WU, Xumeng LIN, Bingjiang CHEN, Qifan ZHOU, Gang XUE. Optimization of key operational parameters in a pyrite driven mixotrophic denitrification system[J]. Industrial Water Treatment, 2026, 46(4): 164-172.

https://gyscl.magtechjournal.com/CN/Y2026/V46/I4/164

图/表 7

图1 各实验组在不同黄铁矿投加量和C/N条件下的出水TN，TN去除率，出水NO₃ ^--N、NH₄ ⁺-N、NO₂ ^--N

Fig.1 Effluent TN，TN removal efficiency，NO₃ ^--N，NH₄ ⁺-N，and NO₂ ^--N of each experimental group under different pyrite dosages and C/N ratios

图2 各实验组在不同黄铁矿投加量和C/N条件下的出水TP和TP去除率

Fig.2 Effluent TP concentration and TP removal efficiency of each experimental group under different pyrite dosages and C/N ratios

图3 不同pH条件下的出水氮素质量浓度和TN去除率

Fig.3 Effluent nitrogen concentration and TN removal efficiency under different pH conditions

图4 不同pH条件下的出水TP和TP去除率

Fig.4 Effluent TP concentration and TP removal efficiency under different pH conditions

图5 不同温度条件下的出水氮素浓度和TN去除率

Fig.5 Effluent nitrogen concentration and TN removal efficiency under different temperature conditions

图6 不同温度条件下的出水TP和TP去除率

Fig.6 Effluent TP concentration and TP removal efficiency under different temperature conditions

图7 不同黄铁矿投加量条件下初始和结束时的MLSS、MLVSS和MLVSS/MLSS

Fig.7 Initial and final MLSS，MLVSS and MLVSS/MLSS under different pyrite dosage conditions

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