摘要：

亚洲首座Nereda^®好氧颗粒污泥污水处理厂（AGS-WWTP）在浙江省龙游县落地，并取得了良好的成效。好氧颗粒污泥（AGS）在长期运行过程中会形成稳定的粒径和元素组成，微生物群落结构发生演变并趋于平衡。为探究实际污水处理厂长期运行中产生的AGS与一般活性污泥（AS）的差异，通过有机元素分析和胞外聚合物（EPS）分析探究了AGS与AS在成分上的差异，通过高通量测序探究了AGS与AS在微生物群落组成上的不同。结果表明，相比于AS，AGS的有机成分相差不大，但是含有更丰富的EPS。AGS含有与AS不同的优势菌种，在AGS形成过程中γ变形菌（Gammaproteobacteria）、亚硝化螺旋菌（Nitrospirota）、Candidatus competibacter等能够进行脱氮的菌群得到富集；AGS中微生物种群多样性明显减少，表明AGS形成过程中会淘汰部分种群，从而降低污泥中微生物种群的多样性，同时功能菌群在此过程中得到富集。

关键词: 好氧颗粒污泥, 微生物群落, 优势菌种, 污水处理厂

Abstract:

Asia’s first Nereda^® aerobic granular sludge sewage treatment plant（AGS-WWTP） was established in Longyou County，Zhejiang Province，and achieved good results. Aerobic granular sludge（AGS） formed stable particle sizes and element compositions during long-term operation，and the microbial community structure evolved and tended to be balance. In order to explore the differences between AGS and general activated sludge（AS） generated in the long-term operation of sewage treatment plants，the difference of the composition of AGS and AS were explored through organic element analysis and extracellular polymers（EPS） analysis. The difference of microbial community structure between AGS and AS were analyzed by the high-throughput sequencing. The results showed that AGS was not much different from AS in organic composition，but contained more abundant EPS. The dominant microbes in AGS and AS were different. During the process of AGS formation，the bacteria groups capable of denitrification such as Gammaproteobacteria，Nitrospirota and Candidatus competibacter were enriched. The diversity of microbial populations in AGS was significantly reduced，indicating that some populations would be eliminated during the formation of AGS，thereby reducing the diversity of microbial populations in sludge. At the same time，the functional flora was enriched during this process.

Key words: aerobic granular sludge, microbial community, dominant bacteria, sewage treatment plant

中图分类号: 

• X703.1