Advances in the microbial flora of anaerobic digestive systems

doi:10.11894/1005-829x.2011.31(10).10

Abstract

Abstract:

A large number of microbes are distributed in various kinds of anaerobic digestive reactors for treating wastewater and wastes. Under anaerobic conditions，they interact and decompose the complicated organisms into carbon dioxide and methane，which are the functional basis of the anaerobic treatment of wastes. The molecular biological technology，which does not depend on cultivation，leads to the discovery of a great number of bacteria and archaea in eco-systems，but most of them have not been cultured successfully，yet，and their ecological functions have not been expounded either. Now，under the operation conditions，such as substrate species，organic loads，pH，temperature，etc. of various anaerobic digestive reactors，the structural characteristics and evolution rules ofmicrobial flora，as well as the progress in the research on the technologies related to in situ functions of un-cultured microbes，are summarized. Furthermore，the potentials of some new technologies，such as stable isotope labeling，metagenomics，proteomics and metabolomics in the research on un-cultured micro-organism functions are predicted.

Key words: anaerobic digestion, microbial flora, un-cultured microorganisms, molecular ecological technology

摘要：

各种污水或污物厌氧消化反应器中分布着大量的微生物，它们在厌氧条件下相互协作将复杂的有机物分解为二氧化碳和甲烷，成为废弃物厌氧处理的功能基础。不依赖于培养的分子生物学技术，使大量的细菌和古菌类群在此生态系统中得以发现，但它们大多数仍未被成功培养，其生态功能也未得到阐明。总结了基质种类、有机负荷、pH、温度等各种厌氧消化反应器运行条件下，微生物群落结构的特点和演化规律，以及未培养微生物的原位功能研究技术的进展，展望了稳定性同位素标记、宏基因组学、蛋白质组学和代谢物组学等新技术在未培养微生物功能研究中的潜力。

关键词: 厌氧消化, 微生物群落, 未培养微生物, 分子生态学技术

CLC Number:

Q938

Liu Junhan, Hu Guangrong, Li Fuli, Li Li. Advances in the microbial flora of anaerobic digestive systems[J]. INDUSTRIAL WATER TREATMENT, 2011, 31(10): 10-14.

刘君寒, 胡光荣, 李福利, 李力. 厌氧消化系统微生物菌群的研究进展[J]. 工业水处理, 2011, 31(10): 10-14.

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URL: https://gyscl.magtechjournal.com/EN/10.11894/1005-829x.2011.31(10).10

https://gyscl.magtechjournal.com/EN/Y2011/V31/I10/10

References

[1] Kaeberlein T，Lewis K，Epstein S S. Isolating “uncultivable” microorganisms in pure culture in a simulated natural environment [J].Science，2002，296(5570)：1127-1129.
[2] Amann R I，Ludwig W，Schleifer K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiol. Rev.，1995，59(1)：143-169.
[3] Talbot G，Topp E， Palin M F，et al. Evaluation of molecular methods used for establishing the interactions and functions of microorganisms in anaerobic bioreactors[J]. Water Res.， 2008，42(3)：513-537.
[4] Ahring B K. Perspectives for anaerobic digestion[J]. Adv. Biochem.Eng. Biotechnol.，2003，81：1-30.
[5] Sekiguchi Y. Yet-to-be cultured microorganisms relevant to methane fermentation processes[J]. Microbes and Environ.，2006，21(1)：1-15.
[6] Juretschko S，Loy A，Lehner A，et al. The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full cycle rRNA approach[J]. Syst. and Appl. Microbiol.，2002，25(1)：84-99.
[7] Hiraishi A，Iwasaki M，Shinjo H. Terminal restriction pattern analysis of 16S rRNA genes for the characterization of bacterial communities of activated sludge[J]. J. Biosci. Bioeng.， 2000，90(2)：148-156.
[8] Ariesyady H D，Ito T，Okabe S. Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester[J]. Water Res.，2007，41(7)：1554-1568.
[9] Mino T. Microbial selection of polyphosphate-accumulating bacteria in activated sludge wastewater treatment processes for enhanced biological phosphate removal [J]. Biochemistry (Mosc)，2000，65(3)：341-348.
[10] Jeon C O，Lee D S，Park J M. Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor[J]. Water Res.，2003，37(9)：2195-2205.
[11] Liu W T，Linning K D，Nakamura K，et al. Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content[J]. Microbiology，2000，146 (5)：1099-1107.
[12] Smith R C，Oerther D B. Microbial community development in a laboratory-scale nitrifying activated sludge system with input from a side-stream bioreactor treating digester supernatant [J]. WaterSci. Technol.，2006，54(1)：209-216.
[13] Chouari R，Le Paslier D，Daegelen P，et al. Novel predominantarchaeal and bacterial groups revealed by molecular analysis of an anaerobic sludge digester [J]. Environ. Microbiol.， 2005，7 (8)：1104-1115.
[14] Cheon J，Hidaka T，Mori S，et al. Applicability of random cloningmethod to analyze microbial community in full-scale anaerobic digesters[J]. J. Biosci. Bioeng.，2008，106(2)：134-140.
[15] Zhang Y H P，Lynd L R. Cellulose utilization by Clostridium thermocellum：bioenergetics and hydrolysis product assimilation [J].Proceeding of the National Academy of Sciences of the UnitedStates of America，2005，102(20)：7321-7325.
[16] Delbes C，Moletta R，Godon J. Bacterial and archaeal 16S rDNAand 16S rRNA dynamics during an acetate crisis in an anaerobicdigestor ecosystem [J]. FEMS Microbiol. Ecol.，2001，35 (1)：1926.
[17] Zhang Tong，Ke Shuizhou，Liu Yan，et al. Microbial characteristicsof a methanogenic phenol-degrading sludge[J]. Water Sci. Technol.,2005，52(1/2)：73-78.
[18] Chachkhiani M，Dabert P，Abzianidze T，et al. 16S rDNA characterisationof bacterial and archaeal communities during start-up ofanaerobic thermophilic digestion of cattle manure [J]. Bioresour.Technol.，2004，93(3)：227-232.
[19] Ki D，Park J，Lee J，et al. Microbial diversity and population dynamicsof activated sludge microbial communities participating inelectricity generation in microbial fuel cells[J]. Water Sci. Technol.，2008，58(11)：2195-2201.
[20] Huang Linan ， de Wever H ， Diels L . Diverse and distinctbacterial communities induced biofilm fouling in membranebioreactors operated under different conditions [J]. Environ. Sci .Technol.， 2008，42(22)：8360-8366.
[21] Leclerc M，Delgenes J P，Godon J J. Diversity of the archaeal communityin 44 anaerobic digesters as determined by single strandconformation polymorphism analysis and 16S rDNA sequencing[J].Environ. Microbiol.，2004，6(8)：809-819.
[22] Sun Yujian，Zuo Jiane，Chen Lili，et al. Eubacteria and Archaeacommunity of simultaneous methanogenesis and denitrificationgranular sludge [J]. J. Environ. Sci. (China)，2008，20 (5)：626-631.
[23] 洪璇，刘彬彬，张晓君，等. 喹啉与吲哚驯化的反硝化反应器的微生物群落结构分析比较[J]. 微生物学报，2008，48(4)：503-507.
[24] Fernandez N，Sierra-Alvarez R，Field J A，et al. Microbial communitydynamics in a chemolithotrophic denitrification reactorinoculated with methanogenic granular sludge[J]. Chemosphere，2008，70(3)：462-474.
[25] Liu W T，Chan O C，Fang H H P. Microbial community dynamicsduring start-up of acidogenic anaerobic reactors [J]. Water Res.，2002，36(13)：3203-3210.
[26] Zhang Tong，Liu Yan，Fang H H P. Effect of pH change on theperformance and microbial community of enhanced biologicalphosphate removal process[J]. Biotechnol. Bioeng.，2005，92(2)：173-182.
[27] Rastogi G，Ranade D R，Yeole T Y，et al. Investigation of methhanogenpopulation structure in biogas reactor by molecular characterizationof methyl-coenzyme M reductase A (mcrA) genes[J].Bioresour. Technol.，2008，99(13)：5317-5326.
[28] Briones A M，Daugherty B J，Angenent L T，et al. Microbial diversityand dynamics in multi- and singl-compartment anaerobic bioreactorsprocessing sulfate-rich waste streams [J]. Environ. Microbiol.，2007，9(1)：93-106.
[29] Adamczyk J，Hesselsoe M，Iversen N，et al. The isotope array， anew tool that employs substrate-mediated labeling of rRNA for determinationof microbial community structure and function [J].Appl. Environ. Microbiol.，2003，69(11)：6875-6887.
[30] Osaka T，Ebie Y，Tsuneda S，et al. Identification of the bacterialcommunity involved in methane-dependent denitrification in activatedsludge using DNA stable-isotope probing[J]. FEMS Microbiol.Ecol.，2008，64(3)：494-506.
[31] Elshahed M S，Najar F Z，Aycock M，et al. Metagenomic analysis ofthe microbial community at Zodletone Spring (Oklahoma)：insightsinto the genome of a member of the novel candidate division OD1[J].Appl. Environ. Microbiol.，2005，71(11)：7598-7602.
[32] Sekiguchi Y，Takahashi H，Kamagata Y，et al. In situ detection，isolation， and physiological properties of a thin filamentous microorganismabundant in methanogenic granular sludges： a novelisolate affiliated with a clone cluster， the green non-sulfur bacteria，subdivision I [ J ] . Appl. Environ. Microbiol. ，2001 ，67 (12 ) ：5740-5749.
[33] Yamada T， Sekiguchi Y， Imachi H， et al. Diversity， localization，and physiological properties of filamentous microbes belonging toChloroflexi subphylum I in mesophilic and thermophilic methanogenicsludge granules [J]. Appl. Environ. Microbiol.，2005，71(11)：7493-7503.
[34] Kim J J，Kim H N，Masui R，et al. Isolation of uncultivable anaerobicthermophiles of the family Clostridiaceae requiring growth-supportingfactors[J]. J. Microbiol. Biotechnol.，2008，18(4)：611-615.
[35] Yoshiteru A，Tomoyuki K，Toru H，et al. Hollow-Fiber membranechamber as a device for in situ environmental cultivation[J]. Appl.and Environ. Microbiol.，2009，75(11)：3826-3833.

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