Ammonia inhibition of anaerobic digestion

doi:10.11894/1005-829x.2010.30（4).9

Abstract

Abstract:

A detailed summary of the research conducted on the ammonia inhibition of anaerobic processes is provided.Due to the difference in anaerobic inoculation， waste composition， and experimental methods and conditions which have been used， the ammonia inhibition efficiency are different as well. The domestication of microorganism and using various methods before anaerobic digestion can cause the ammonia to remove or counteract toxicity with other toxicants so as to significantly improve the waste treatment efficiency.

Key words: anaerobic digestion, ammonia inhibition, wastewater treatment, agricultural waste, industrial waste

摘要：

对厌氧消化工艺氨抑制的相关研究进展作了综述，对氨抑制的机制进行了剖析，对其影响因素进行了总结，并提出了工程对策。结果表明，由于所用接种污泥、废物成分、试验条件和方法各不相同，氨抑制效应也各异。微生物驯化以及在厌氧消化前采取多种方法，可促成氨的去除或与其他毒物相互抵消毒性，从而显著改善废物处理的效率。

关键词: 厌氧消化, 氨抑制, 废水处理, 农业废物, 工业废物

CLC Number:

X703.1

Jin Rencun, Huang Guannan, Ma Chun, Yang Guangfeng. Ammonia inhibition of anaerobic digestion[J]. INDUSTRIAL WATER TREATMENT, 2010, 30(4): 9-12.

金仁村, 黄冠男, 马春, 阳广凤. 厌氧消化工艺的氨抑制现象[J]. 工业水处理, 2010, 30(4): 9-12.

/ / Recommend / Download Citations

URL: https://gyscl.magtechjournal.com/EN/10.11894/1005-829x.2010.30（4).9

https://gyscl.magtechjournal.com/EN/Y2010/V30/I4/9

References

［1］ Kelleher B P，Leahy J J，Henihan A M，et al. Advances in poultry litter disposal technology － a review［J］. Bioresour. Technol.，2000，83（1）：27－36.
［2］ Ghosh S，Pohland F G. Kinetics of substrate assimilation and product formation in anaerobic digestion［J］.J.Water Pollut. ControlFed.，1974，46（4）：748－759.
［3］ Tchobanoglous G，Theisen H，Vigil S. Integrated waste management：Engineering principles and management issues［M］. New York：McGraw-Hill，Book Co.，1993.
［4］ Whittmann C， Zeng A P， Deckwer W D. Growth inhibition by ammonia and use of pH-controlled feeding strategy for the effective cultivation of mycobacterium chlorophenolicum ［J］.Appl. Microbiol. Biotechnol.，1995，44（3／4）：519－525.
［5］ de Baere L A，Devocht M，van Assche P， et al. Influence of high NaCl and NH₄Cl salt levels on methanogenic associations ［J］.Water Res.， 1984， 18（5）：543－548.
［6］ Gallert C，Bauer S，Winter J.Effect of ammonia on the anaerobicdegradation of protein by a mesophilic and thermophilic biowaste population［J］.Appl. Microbiol. Biotechnol.，1998，50（4）：495－501.
［7］ Kayhanian M.Performance of a high-solids anaerobic digestion process under various ammonia concentrations［J］.J.Chem.Tech.Biotechnol.，1994，59（5）：349－352.
［8］ Koster I W，Lettinga G.Anaerobic digestion at extreme ammonia concentrations［J］.Biol.Wastes，1988，25（1）：51－59.
［9］ Calli B， Mertoglu B，Inanc B，et al. Effects of high ammonia concentrations on the performances of anaerobic bioreactors ［J］.Process Biochem.，2005，40（3／4）：1285－1292.
［10］ Wiegant W M，Zeeman G.The mechanism of ammonia inhibition in the thermophilic digestion of livestock wastes［J］.Agric. Wastes，1986，16（3）：243－253.
［11］ Jarrell K F，Saulnier M，Ley A. Inhibition of methanogenesis in pure cultures by ammonia，fatty acids，and heavy metals，and protection against heavy metal toxicity by sewage sludge ［J］.Can.J. Microbiol.，1987，33（6）：551－555.
［12］ Liu T，Sung S.Ammonia inhibition on thermophilic aceticlastic methanogens［J］.Water Sci. Technol.，2002，45（10）：113－120.
［13］ van Velsen A F M.Adaptation of methanogenic sludge to highammonia－nitrogen concentrations［J］.Water Res.，1979，13（3）：995－999.
［14］ Braun B，Huber P，Meyrath J.Ammonia toxicity in liquid piggery manure digestion［J］.Biotechnol. Lett.，1981，3（4）：159－164.
［15］ Parkin G F，Miller S W.Response of methane fermentation to continuous addition of selected industrial toxicants［C］//Proceedings of the 37th Purdue Industrial Waste Conference.1982：729-743.
［16］ Hashimoto A G.Ammonia inhibition of methanogenesis from cattle waste［J］. Agric.Wastes，1986， 17（4）：241－261.
［17］ Hendriksen H V，Ahring B K.Effects of ammonia on growth and morphology of thermophilic hydrogen-oxidizing methanogenic bacteria［J］.FEMS Microb. Ecol.，1991，85（3）：241－246.
［18］ Angelidaki I，Ahring B K.Anaerobic thermophilic digestion of manure at different ammonia loads：effect of temperature ［J］.Water Res.，1994，28（3）：727－731.
［19］ Soubes M， Muxi L，Fernandez A，et al. Inhibition of methanogenesis from acetate by Cr3＋ and ammonia［J］.Biotechnol.Lett.，1994，16（2）：195－200.
［20］ Borja R，Sanchez E，Weiland P.Influence of ammonia concentration on thermophilic anaerobic digestion of cattle manure in upflow anaerobic sludge blanket（UASB） reactors［J］. Process Biochem.，1996，31（5）：477－483.
［21］ Boardman G D，McVeigh P J. Use of UASB technology to treat crab processing wastewaters［J］.J.Environ. Eng.，1997，123（8）：776－785.
［22］ Gallert C， Winter J.Mesophilic and thermophilic anaerobic digestion of source-sorted organic waste：effect of ammonia on glucose degradation and methane production［J］.Appl.Microbiol.Biotechnol.，1997，48（3）：405－410.
［23］ Guerrero L，Omil F，Mendez R，et al.Treatment of saline wastewaters from fish meal factories in an anaerobic filter underextreme ammonia concentrations ［J］.Bioresour. Technol.，1997，61（1）：69－78.
［24］ Krylova N I，Khabiboulline R E，Naumova R P，et al. The influence of ammonium and methods for removal during the anaerobic treatment of poultry manure［J］.J.Chem.Tech.Biotechnol.，1997，70（1）：99－105.
［25］ Poggi-Varaldo H M，Rodriguez-Vazquez R，Fernandez-Villagomez G，et al.Inhibition of mesophilic solid-substrate anaerobic digestion by ammonia nitrogen［J］.Appl.Microbiol.Biotechnol.，1997，47（3）：284－291.
［26］ Chamy R，Poirrier P，Schiappacasse M C，et al.Effect of ammonia content in the biodegradability of the salmon industry wastes ［J］.Bioprocess Eng.，1998，19（1）：1－5.
［27］ Hansen K H，Angelidaki I，Ahring B K.Anaerobic digestion of swine manure：inhibition by ammonia［J］.Water Res.，1998，32（1）：5－12.
［28］ Bujoczek G，Oleszkiewicz J，Sparling R，et al. High solid anaerobic digestion of chicken manure［J］.J.Agric.Eng.Res.，2000，76（1）：51－60.
［29］ Sung S，Liu T.Ammonia inhibition on thermophilic digestion ［J］.Chemosphere，2003，53（1）：43－52.
［30］ Hansen K H，Angelidaki I，Ahring B K.Improving thermophilic anaerobic digestion of swine manure［J］.Water Res.，1999，33（8）1805－1810.
［31］ Angelidaki I，Ellegaard L，Ahring B K.A mathematical model for dynamic simulation of anaerobic digestion of complex substrates：focusing on ammonia inhibition［J］.Biotechnol. Bioeng.，1993，42（2）：159－166.
［32］ Zeeman G，Wiegant W M，Koster-Treffers M E，et al. The influence of the total ammonia concentration on the thermophilic digestion of cow manure［J］.Agric. Wastes，1985，14（1）：19－35.
［33］ McCarty P L，McKinney R E.Salt toxicity in anaerobic digestion ［J］.J.Water Pollut.Control Fed.， 1961， 33（3）： 399－415.
［34］ Kugelman I J， McCarty P L. Cation toxicity and stimulation in anaerobic waste treatment［J］.J.Water Pollut.Control Fed.，1964，37（1）：97－116.
［35］ Melbinger N R，Donnellon J.Toxic effects of ammonia－nitrogen in high rate digestion［J］.J.Water Pollut.Control Fed.，1971，43（8）：1658－1670.
［36］ Speece R E.Anaerobic biotechnology for industrial waste treatment［J］.Environ. Sci.Technol.，1983，17（9）：416－427.
［37］ Parkin G F，Speece R E， Yang C H J，et al. Response of methane fermentation systems to industrial toxicants ［J］.J.Water Pollut.Control Fed.，1983，55（1）：44－53.
［38］ Kabdasli I，Tunay O，Ozturk I，et al. Ammonia removal from young landfill leachate by magnesium ammonium phosphate precipitation and air stripping［J］.Water Sci.Technol.，2000，41（1）：237－240.
［39］唐崇俭，郑平，金仁村，等.猪场废水厌氧生物处理FAN 抑制及其调控对策的研究［J］.高校化学工程学报，2008，22 （4）：697 －702.
［40］ Callaghan F J，Wase D A J，Thayanithy K，et al.Codigestion of waste organic solids：batch studies［J］.Bioresour.Technol.，1999，67（2）：117－122.
［41］ Nielsen H B， Angelidaki I.Strategies for optimizing recovery of the biogas process following ammonia inhibition ［J］.Bioresour.Technol.，2008， 99（17）：7995－8001.
［42］何仕均，王建龙，赵璇. 氨氮对厌氧颗粒污泥产甲烷活性的影响［J］.清华大学学报：自然科学版， 2005，45（9）：1294－1296.
［43］ Hanaki K，Hirunmasuwan S，Matsuo T.Protection of methanogenic bacteria from low pH and toxic materials by immobilization using polyvinyl alcohol［J］.Water Res.，1994，28（4）：877－885.
［44］ Borja R，Sanchez E，Duran M M. Effect of the clay mineral zeolite on ammonia inhibition of anaerobic thermophilic reactors treating cattle manure ［J］.J.Environ. Sci.Health A，1996,31（2）：479－500.

[1]	Xiaojun MENG, Guanghuan GE, Haiwen CHAI. Carbon reduction strategies in the green transition of wastewater treatment plants [J]. Industrial Water Treatment, 2026, 46(4): 25-34.
[2]	Peihong ZHAO, Gaoli ZHAO, Chenkai LÜ, Haodong ZHAN, Haobo WANG, Zhou YU, Jian WANG, Meng HUANG, Changsong MEI. Research and practice on energy conservation and consumption reduction of the aeration system in a wastewater treatment plant in southern China [J]. Industrial Water Treatment, 2026, 46(3): 201-208.
[3]	Ying WU, Huanghuan XU, Yingqiao LONG, Yushi WANG, Qiuping WEI, Qunhuan CAI, Gang XUE. Electrochemical treatment of dimethyl sulfoxide industrial wastewater by boron doped diamond electrode [J]. Industrial Water Treatment, 2026, 46(3): 57-64.
[4]	Huasheng WANG, Fengqiu ZHOU, Zhenning ZHOU, Dan CHEN, Jiaqi LI. Study on the treatment performance of high ammonia nitrogen tail water from rare earth mines by acid-tolerant microalgae with adherent culture [J]. Industrial Water Treatment, 2026, 46(3): 65-73.
[5]	Tao LIN, Yiling ZHAO, Yongchang XUE. Research progress of bioaugmentation technology in wastewater treatment [J]. Industrial Water Treatment, 2025, 45(9): 39-47.
[6]	Feiyu HE, Ling FENG, Ming ZHU, Li CHEN, Binghui TIAN, Xueke WANG, Yongzhi CHI, Cuilian FU. Advances in selective separation and recovery of valuable substances from industrial wastewater by electrodialysis [J]. Industrial Water Treatment, 2025, 45(9): 10-18.
[7]	Sen WANG, Shaohua TONG, Xiaojun GAO, Xiaoping JING, Wenfeng WANG, Hongfang SUN. Research progress of microelectrolysis technology for industrial wastewater treatment [J]. Industrial Water Treatment, 2025, 45(9): 29-38.
[8]	Anna WANG, Shilong XU, Minzhong YUAN, Weifang YUAN, Feng JIANG, Lu YAO. Research progress on sulfur-based composite filler applied to denitrification of wastewater [J]. Industrial Water Treatment, 2025, 45(8): 11-18.
[9]	Liangcai LIU, Wenyu MAO, Yijie ZHENG, Zejun DAI, Qixing HU, Zhiquan HU, Peng CHEN, Jun ZHENG, Likan LIU. Advances in the application of optimization algorithms in wastewater treatment [J]. Industrial Water Treatment, 2025, 45(7): 11-18.
[10]	Xinrui ZHANG, Weinan GAO, Yiren WANG, Cuimin FENG, Shuhui CHENG, Haixia JI. Design and analysis of sludge treatment system for a comprehensive wastewater treatment plant [J]. Industrial Water Treatment, 2025, 45(7): 200-205.
[11]	Rui LUO, Guoyuan LEI, Shunming ZHOU, Xuewen HU, Da ZHOU, Xiao MA, Si WU. Pilot study on the removal of hardness of reverse osmosis concentrated water in steel enterprises by crystallized fluidized bed [J]. Industrial Water Treatment, 2025, 45(6): 179-186.
[12]	Xinwei ZHOU, Xiangzhuang FU, Mengxia GUO, Wen XU, Dexin WANG. Treatment of protein-containing organic wastewater by “electrical” collaboration between dissimilatory iron reduction and methanogenesis [J]. Industrial Water Treatment, 2025, 45(6): 167-178.
[13]	Xinping HUANG, Qian ZHAO, Jianhua CHEN, Rui MA, Lei ZHAO, Nayu WANG, Yan ZHANG. Construction of wastewater traceability and early warning system in industrial park based on three-dimensional fluorescence spectroscopy [J]. Industrial Water Treatment, 2025, 45(6): 234-242.
[14]	Lin CHEN, Xin YAN, Zhihe TANG, Zhaokuan RAN, Binlian LI, Hui LUAN, Chunmao CHEN. Water quality prediction model of air flotation unit based on optimization support vector regression machine [J]. Industrial Water Treatment, 2025, 45(5): 157-165.
[15]	Jie TIAN, Li LIU, Rong LIU, Lu LIU, Peijun ZOU, Jiangrong CHEN, Mo CHEN, Yiwen ZHANG, Fusen YAO, Gang ZHANG, Li GU. Carbon flow direction analysis of inverted A²O wastewater treatment process [J]. Industrial Water Treatment, 2025, 45(5): 177-182.

Please choose a citation manager

Content to export