摘要：

随着石油及石油产品的广泛使用，地下水石油烃污染日益加重。原位可渗透反应墙（PRB）技术是修复地下水污染的重要手段。基于室内PRB模拟柱实验，采用生物炭固定化石油烃降解菌作为PRB反应介质，探究了PRB装置的最适地下水流量，并初步考察了微生物PRB反应介质的性能。结果表明，微生物PRB装置的最适用地下水流量为6 mL/min，运行30 d对自配废水和实际污染场地地下水石油烃的去除率分别为88.41%和69.72%；随反应进行，出水pH无显著变化，氧化还原电位和溶解氧浓度降低。微生物PRB反应介质性能评价结果表明，该PRB反应介质对直链烷烃（C10~C25）的降解效果较好；对吸附后的固定化菌进行解吸，石油烃析出较为迅速，解吸量为53.66 mg/g。

关键词: 地下水, 石油烃污染, 原位PRB技术, 固定化微生物

Abstract:

With the extensive use of petroleum and petroleum products, petroleum hydrocarbon pollution in groundwater becomes serious. In-situ permeable reactive wall(PRB) is an important means to remediate groundwater pollution. Based on the PRB simulation column experiments, biochar-immobilized petroleum hydrocarbon-degrading bacteria as the PRB reaction medium were used to explore the optimum groundwater flow rate, and the performance of the microbial PRB reaction medium was investigated. The results showed that the optimal groundwater flow rate of the microbial PRB was 6 mL/min, and the removal rates of petroleum hydrocarbons in the simulated wastewater and the polluted groundwater were 88.41% and 69.72% after 30 days, respectively. With the running of reaction, the pH of the effluent did not change significantly, while the redox potential and content of dissolved oxygen decreased. The performance evaluation of microbial PRB reaction medium during the reaction showed that the PRB reaction medium had effective degradation on straight-chain alkanes (C10-C25). The desorption of petroleum hydrocarbons precipitate from the immobilized bacteria was relatively rapid, with a desorption amount of 53.66 mg/g.

Key words: groundwater, petroleum hydrocarbons contamination, in-situPRB, immobilized microorganism